产品货号：
GS2158
中文名称：
FauI内切酶
英文名称：
FauI
产品规格：
50U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
相关搜索：FauI内切酶，FauI限制性内切酶
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | CCCGC(N)4↑ |
| 来源 | E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili |
| Assayed on | Lambda DNA |
| Optimal buffer | B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.) |
| Optimal temperature | 55℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycero;Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and of these 95%can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 4 u.a.of enzyme for 16hoursat 55℃ |
| Methlation effect | Blocked by CG methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | SmuI |
相关搜索：FauI内切酶，FauI限制性内切酶